CULTIVATING SUCCESS THROUGH SCIENCE

Transcript

1 INSTRUCTION

2 CULTIVATING SUCCESS THROUGH SCIENCE For more than 20 years, VICAM has been the global provider of choice for next-generation food safety technology and rapid mycotoxin test solutions. The goal of securing the world s food supply requires knowledge, cooperation, and collaboration. To advance that objective, VICAM works closely with government regulatory agencies, international standards bodies, major food industry laboratories, and leading research institutions around the world. With a global scientific and distribution presence, VICAM has access to the latest research advances, industry expertise, and regulatory information needed to design solutions that maximize food safety and quality at every stage of the global food supply chain. As the trusted partner of the agricultural industry, VICAM is proud to consistently deliver the superior products, responsive service, and individualized support for your industryspecific goals. 2

3 Afla-V Strip Tests AQUA Solution A AQUA Solution B Filter Paper Afla-V AQUA Europe Barcode..

4 FAST, PRECISE AND SOLVENT-FREE 2 4

5 VICAM Afla-V AQUA answers one of today s toughest food safety challenges with a simple, water-based aflatoxin test that delivers precise quantitative results in just minutes while eliminating the risk and cost associated with on-site solvent use and disposal. Aflatoxin is the toxic byproduct of several mold species which are natural inhabitants of soil. Classified as a Group I carcinogen by the International Agency for Research on Cancer (IARC), aflatoxin is responsible for serious economic losses due to animal disease and reduced production efficiency. Afla-V stri tests utilize the proven sensitivity and selectivity of VICAM s monoclonal antibodies to accurately detect and measure total aflatoxins B 1, B 2, G 1 and G 2 at levels as low as 2 ppb and as high as 40 ppb. Afla-V AQUA eliminates the need for hazardous solvents using a water-based, dilution-free extraction procedure that develops in 5 minutes. Digital results are clearly displayed on the screen of the Vertu Lateral Flow 3

6 AFLA-V AQUA PROCEDURE CAMPIONE MAIS STRUMENTAZIONE Vertu Reader SET UP 1. Calibrate il VERTU READER quotidianamentescannerizzandoilbar-code AFLA AQUA. Contraollare che sul display dellettorecompaiailnumerodellottodelgi strip che state utilizzando ESTRAZIONE CAMPIONE 1. Pesare 5 gr di campione finemente macinato nella provetta di estrazione. 2. aggiungere al campione nella provetta 15mL di acqua distillata (deionizzata). + 5 ml di AQUA Solution A (cod ) + 5 ml of AQUA Solution B (cod ) 3. Sigillarelaprovettaeagitarlatramitevortex alla massima velotcità per 2 minuti. 4. Filtrare l estratto. (cod ) STRIP TEST 1. Trasferire 100 µl della solutzione di estrazionenell aperutracircolaredell Afla-V strip ad una velocita di circa 1 goggia al secondo. 2. Aspettarechelostripsisviluppiper5minuti 3. inserire Afla-V strip test nel Vertu reader (l apertura circolare dello strip verso l interno). Strip tests devono essere conservati a una temperatura di 4 8 C (39 46 F). Strip test e l estrazion del campione devono avvenire a una temperatura compresa tra 20 C (68 F) and 25 C (77 F). Attenersi alla procedura VICAM.Analizzare solamanente campioni alimentari. I prodotti VICAM non vanno ingeriti o apllicati sulla pelle 4 6

7 4. Premere i l bottone centrale e attendere che lo strumento legga lo strip 5. Per stampare il risultato, spostare il cursose su P e premere il bottone centrale. 6. Per leegere un nuovo campione, spostare il cursore su NT e premere il bottone centrale. RANGE 0-40 ppb LIMIT OF DETECTION >2 ppb Benefici: NO solventi Velocità: Resultati in 5 minuti* Sensibilità: Limits of detection > 2ppb Scadenza Strips : circa 10 mesi Range: 0-40 ppb NOTES 1. Durante la scansione del codice a barre, assicurarsi che il + rosso dello scanner sia al centro del codice a barre. L altezza dello scanner dal codice a barre deve essere di crica 20 cm 2. Premere il pulsante centrale dello strumento per leggere i campioni. Premendo il tasto centrale della tastiera per più di 3 seconti avverà lo spegnimento dello strumento 3. Utilizzare esclusivamente acqua distillata o deionizzata 4. Tutteigli strip utilizzati devono avere la linea di controllo * after extraction 5

8 DISTRIBUORE: SAFE FOOD Via Paolo Ponzoni 13/A PARMA Tel.: Fax: Subject to change without notice. 2015WatersCorporation.Waters,TheScienceofWhat spossible,vicam,afla-vandvertuareregisteredtrademarksof Waters Corporation. AQUA and Cultivating Success Through Science are trademarks of Waters Corporation. TheanalyticalmethodspresentedinthisdatasheethavebeenresearchedanddevelopedbyVICAMtobeusedexclusivelywithAfla-VAQUAproducts.Thesemethodshave beenvalidatedinthevicamlaboratoriestoperformtothespecificationsindicatedintheafla-vaquaprocedures.theuserassumesallriskinusingafla-vaquaprocedures andproducts.vicammakesnowarrantyofanykind,expressedorimplied,otherthanthatafla-vaquaproductsconformtovicam sprintedspecificationandqualitycontrol standards.vicamwill,atitsoption,repairorreplaceanyproduct,orpartthereof,whichprovestobedefectiveinworkmanshipormaterial.vicam sundertakingtorepairor servicesuchproductsisexclusiveandisinlieuofallotherwarrantieswhetherwritten,oral,expressed,orimplied,includinganyimpliedwarrantyofmerchantabilityorfitness foraparticularpurpose.vicamshallhavenoliabilityforanticipatedorlostprofitsoranyloss,inconvenienceordamagewhetherdirect,indirect,incidental,consequentialor otherwise, to person or property, or for strict liability or negligence arising from or in connection with the use of these assay procedures or Afla-V AQUA products EN August 2015 AW-POD