Rosalinda Madonna, MD, PhD CURRICULUM VITAE

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1 Rosalinda Madonna, MD, PhD CURRICULUM VITAE Indirizzo di residenza: Via Armellini, Chieti, Italy Indirizzo professionale: Istituto di Cardiologia, Università degli Studi G. d Annunzio Chieti, c/o Ospedale S. Camillo de Lellis, Via Forlanini 50, Chieti, Italy Tel: ; Fax: Educazione: Laurea in Medicina e Chirurgia, Università degli Studi G. d Annunzio, Chieti (1990-marzo 1997) Curriculum degli studi: voto di Laurea 110/110 e lode, 56 esami sostenuti secondo Tabella XVIII della riforma degli Studi di Medicina, media dei voti riportati: 29,7 Discussione della Tesi di Laurea Sperimentale: " Hind III lipoproteinlipase polimorfism and coronary artery disease, Relatore Prof. A. Barsotti, Controrelatore Prof. A. Procopio. Esame di Stato per l Iscrizione all Albo Professionale medico di Chieti Medical Board of Chieti, Italy (settembre 1997) Università degli Studi G. d Annunzio, Chieti, Dottorato di Ricerca in Tecnologie Biomediche e Bioimmagini Funzionali ad indirizzo cardiologico, XV CICLO, Mentore scientifico per la formazione alla Ricerca Prof. Raffaele De Caterina Febbraio 2003: discussione della tesi di dottorato: Regolazione dell espressione citochino-indotta del gene ossido nitrico sintetasi inducibile in cardiomioblasti. Università degli Studi G. d Annunzio, Chieti, Classe , classificata prima all esame di ammissione alla Scuola di Specializzazione in Cardiologia, Direttore: Prof. A. Barsotti, interrotta per il completamento degli studi di Dottorato di Ricerca Texas University Health Science at Houston, TX (Settembre 2002 Settembre 2004) Post-doctoral Research Fellow, presso il Center for Cardiovascular Biology and Atherosclerosis Research, Mentore Prof. Yong-Jian Geng Texas Heart Institute at St. Luke s Hospital, Houston TX (Settembre 2002 Settembre 2004 ) Post-doctoral Research Fellow, presso Stem Cell and Heart Failure Laboratory, Mentore Prof. Yong-Jian Geng Università degli Studi G. d Annunzio, Chieti, Classe , classificata prima all esame di ammissione alla Scuola di Specializzazione in Cardiologia, Direttore: Prof. R. De Caterina Iscritta al secondo anno della Facoltà di Scienze Biologiche, Università degli Studi dell Aquila. Esperienze in ambito universitario, lavorativo e corsi di formazione: Istitutional animal training course (marzo 2003), presso la University of Texas-Houston Health Science Center, TX Istitutional hazardous chemical safety training course (settembre 2003), presso la University of Texas-Houston Health Science Center, TX Istitutional radioactive safety training course (gennaio 2004), presso la presso la University of Texas-Houston Health Science Center, TX University of Texas Medical School at Houston, Texas (2003) Ha seguito 1 studente di Medicina per il programma di summer research in tema di Cellule Staminali applicate alla biologia cardiovascolare. Università degli Studi G. d Annunzio, Chieti (2000) Ha preso parte come istruttore a lezioni di Semeiotica Medica cardiovascolare per studenti di medicina, presso il reparto di Cardiologia, Ospedale S. Camillo de Lellis, Chieti Università degli Studi G. d Annunzio, Chieti (2000) Ha seguito 2 studenti di Medicina nella preparazione della tesi di Laurea in tema di Aterosclerosi e Diabete Servizio di Guardia Medica estiva ed invernale presso la ASL Lanciano-Vasto (giugno-agosto 1998; giugno-agosto 1999; giugno-agosto 2000; giugno-agosto 2001; impegno non continuativo nei mesi invernali degli anni 2001 e 2002) Università degli Studi G. d Annunzio, Chieti ( ) Studente interno, Istituto di Scienze Cardiovascolari, Dir. Prof. A. Barsotti Università degli Studi G. d Annunzio, Chieti ( ) Corso annuale di Perfezionamento in Ecografia Interna, Dir. Prof. Marzio Università degli Studi G. d Annunzio, Chieti ( ) Tirocinio volontario di Ricerca presso i Laboratori di Farmacologia (Dir. Prof. Caciagli), Genetica Medica (Dir. Prof. Palka) e Patologia Generale ( Dir. Prof. A. Procopio) Università degli Studi dell Aquila ( ) Studente Interno, Reparto di Medicina Interna, Dir. Prof. A. Santucci Università degli Studi dell Aquila ( ) Tirocinio obbligatorio presso il Laboratorio di Patologia Generale, Dir. Prof. Gulino

2 Membro di Società Scientifiche: Membro ordinario della Società Italiana di Cardiologia (2001-presente) Membro ordinario della Società Italiana di Trombosi ed Emostasi (2002- presente) Membro ordinario della European Association Study of Diabetes (EASD, presente ) Premi di Ricerca e Fellowship: 2002: Young Investigator Award, Società Italiana Studio Trombosi ed Emostasi (SISET) 2003: Young Investigator Award, International Society of Thrombosis and Haemostasis : Post-doctoral Research Fellowship, University of Texas Medical School, Houston TX 2004: Travel Grant Award for Young Scientists, 1 st Workshop on Adipose Tissue-derived Stem Cells, Tulane University Health Science Center, New Orleans LA, USA june : Young Investigator Award, European Association Study of Diabetes (EASD) 2004: Young Investigator Award, Società Italiana Studio Trombosi ed Emostasi (SISET) Supporti per la Ricerca: NHI/NHLB1 P.I.: Prof. Geng, Yong-Jian Human embryonic stem cell growth and apoptosis in high density cultures Fondi per la Ricerca, Centro Scienze dell Invecchiamento (CESI) e Consorzio Italiano Ricerche Cardiovascolari (C.I.R.C.), P.I.: Prof. Raffaele De Caterina Fondi per la Ricerca, Centro Scienze dell Invecchiamento (CESI) e Consorzio Italiano Ricerche Cardiovascolari (C.I.R.C.), P.I.: Prof. Raffaele De Caterina Relazioni ad invito: 1. January 2003, Seminar Series of Vascular Biology, University of Texas Medical School at Houston: Inhibition of 3- hydroxy-3-methylglutaryl coenzyme A reductase attenuates expression of inducible nitric oxide synthase in cardiac myoblasts. Possible link to direct myocardial protection during ischemia-reperfusion. 2. January 2004, Seminar Series of Vascular Biology, University of Texas Medical School at Houston: Adipose tissue derived stem cells: new concepts in cardiovascular cell therapy. 3. November 2004, Seminar Series del Centro Scienze dell Invecchiamento, CESI presso l Università degli Studi di Chieti, Italy: Cellule staminali adulte derivate da tessuto adiposo per la rigenerazione cardiaca: caratterizzazione, meccanismi, dati animali e potenzialità. Partecipazione a conferenze nazionali ed internazionali come presenting author di contributi scientifici: 1. M Soccio, R Madonna, A Taccardi, A De Finis, G D Apolito, F Romanazzi, D Statile, P Di Napoli, S Gallina, A Barsotti. Polimorfismo del gene di conversione dell angiotensina ed aterosclerosi carotidea nella popolazione con coronaropatia severa e popolazione di controllo. 59 Congresso Nazionale della Società Italiana di Cardiologia, Roma, Italy december Oral communication. 2. R Madonna, M Soccio, G D Apolito, P Perfetti, P Tiloca, M Zimarino, S Gallina, A Barsotti. Relazione fra malattia coronarica ed aterosclerosi aortica e carotidea. Fattori di rischio comuni?. 60 Congresso Nazionale della Società Italiana di Cardiologia, december 1999 Roma Italy. Oral commnication 3. R Madonna, A Pandolfi, M Massaro, A Consoli, R De Caterina: Insulin enhances vascular cell adhesion molecule (VCAM-1) expression in human cultured endothelial cells. A link to the pathogenesis of accelerated atherosclerosis in diabetes. XIX Congress of the International Society on Thrombosis and Haemostasis, Birmingham UK July Oral communication, Young Investigator Award. 4. R Madonna, P Di Napoli, A Grilli, M Felaco, Y-J Geng, R De Caterina: Inhibition of 3-hydroxy-3-methylglutaryl coenzyme A reductase attenuates expression of inducible nitric oxide synthase in cardiac myoblasts. Possible link to direct myocardial protection during ischemia-reperfusion Research Day, The graduate and postgraduate research forum, Excellence in Biomedical Research, 9 th Annual Event November , poster 5. Madonna R, Li S, Lin J, Rajabi M, Wassler M J, Geng Y-J: Characterization and heart transfusion of stromal cell progenitors isolated from adipose tissue 2003 Research Day, The graduate and postgraduate research forum, Excellence in Biomedical Research, 9 th Annual Event November , Houston TX oral presentation, abstract selected among first 6 finalist abstracts for awarding. 6. R Madonna, P Di Napoli, A Grilli, M Felaco, Y-J Geng, R De Caterina: Inhibition of 3-hydroxy-3-methylglutaryl coenzyme A reductase attenuates expression of inducible nitric oxide synthase in cardiac myoblasts. Possible link to direct myocardial protection during ischemia-reperfusion. Journal of American College of Cardiology 2004, march 3 vol. 43 suppl A rd Annual Scientific Session, New Orleans La, USA march , featured poster with presentation

3 7. R Madonna, Li S, Lin J, Rajabi M, Wassler M J, Geng Y-J: Characterization and heart transfusion of stromal cell progenitors isolated from adipose tissue. 1 st Workshop on Adipose Tissue-derived Stem Cells, Tulane University Health Science Center, New Orleans LA, USA june R Madonna, R De Caterina, J T Willerson, YJ Geng: Adult Mesenchymal Stem Cells Derived from Adipose Tissue Highly Diabetologia suppl. September 2004; International Congress of European Association Study of Diabetes (EASD), Munich 5-7 September 2004, Oral communication 9. R Madonna, Li S, Lin J, Rajabi M, Wassler M J, R De Caterina, Geng Y-J: Characterization and Heart Trasfusion of Endothelial Progenitor Cells isolated from Adipose Tissue. Eur Heart J suppl august 2004, International Congress of European Society of Cardiology Munich 28 august-2 september 2004, e-poster 10. R Madonna, M Massaro, P Di Napoli, A Grilli, M Felaco, Y-J Geng R De Caterina: Inhibition of 3-HMG-CoA reductase attenuates the expression of inducible nitric oxide synthase in cardiac myocytes A link to myocardial protection during ischemia-reperfusion. Eur Heart J suppl august 2004, International Congress of European Society of Cardiology Munich 28 august-2 september 2004, e-poster 11. R Madonna, R De Caterina, M J Wassler, YJ Geng: Telomerase activity and expression in adipose tissue-derived stromal cells Evidences for the existence of a more juvanile phenotype in adipose tissue. Eur Heart J suppl august 2004, International Congress of European Society of Cardiology Munich 28 august-2 september 2004, e-poster 12. R Madonna, R De Caterina, J T Willerson, YJ Geng: Adult Mesenchymal Stem Cells Derived from Adipose Tissue Highly Hematologica suppl October 2004, Congresso Nazionale della Società Italiana per lo Studio della Trombosi ed Emostasi, Roma 30 settembre-3 ottobre, oral communication. 13. R Madonna, M Massaro, P Di Napoli, A Grilli, M Felaco, Y-J Geng R De Caterina: Inhibition of 3-HMG-CoA reductase attenuates the expression of inducible nitric oxide synthase in cardiac myocytes A link to myocardial protection during ischemia-reperfusion. Hematologica suppl October 2004, Congresso Nazionale della Società Italiana per lo Studio della Trombosi ed Emostasi, Roma 30 settembre-3 ottobre, oral communication. 14. R Madonna, Li S, Lin J, Rajabi M, Wassler M J, R De Caterina, Geng Y-J: Expression of nitric oxide synthase during differentiation of telomerase-expressing endothelial cell progenitor derived from adipose tissue. 65 Congresso Nazionale della Società Italiana di Cardiologia, december 2004 Roma Italy. Oral communication. 15. R Madonna, R De Caterina, J T Willerson, YJ Geng: Cardiomyogenic development of telomerase-expressing mesenchymal stem cells from adult adipose tissue. 65 Congresso Nazionale della Società Italiana di Cardiologia, december 2004 Roma Italy. Oral communication. 16. R Madonna, M Massaro, P Di Napoli, A Grilli, M Felaco, A De Caterina, D Tang, Y-J Geng, R De Caterina: Statins enhance differentiation of embryonic stem cells into cardiac myocytes and provide resistance against oxidative stress - A link to the myocardial protection durino ischemia-reperfusion. 65 Congresso Nazionale della Società Italiana di Cardiologia, december 2004 Roma Italy. Oral communication. Linee di Ricerca seguite: Progetti di ricerca pre-dottorato: Genetic epidemiology of coronary artery disease related polymorphisms, including lipoprotein lipase Hind III, PVU II, SER 447 -TER, angiotensin converting enzyme (insertion/deletion) and angiotensin receptor A 1166 C, methilenetetrahidropholate reductase (C 677 -T), endothelial nitric oxide synthase (Glu 298 -Asp) and p22phox C 242 -T; In vivo study of the neuroprotective action of hypoxanthine derivative AIT-082 in NMDA-induced rat striatal and hippocampal lesions. Ex vivo study of inducible nitric oxide synthase (inos) expression in ischemic reperfused rat myocardium, treated with simvastatin, verapamil and adenosine. Durante il dottorato di ricerca: In vitro study of the effects of insulin, glucose and advanced glicosylation products (AGEs) on the endothelial expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) ed E-selectin. In vitro study of the effects of HMG CoA-reductase inhibition on the expression of inducibile nitric oxide synthase (inos) in rat cultured cardiomyocytes. In vitro study of the expression of endothelial nitric oxide synthase in insulin sensitive and insulin resistants (with insulin substrate receptor G 972 R mutation) fibroblasts and HUVEC. In vitro study of the effects of HMG CoA-reductase inhibition by simvastatin on the endothelial expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) ed E-selectin. Durante il research fellowship negli Stati Uniti in vitro study of adult and embryonic stem cell differentiation and proliferation with cardiovascular in vivo applications. ESPERIENZE DI LABORATORIO: Università di Chieti, Istituto di Cardiologia, Laboratorio di Cardiologia Sperimentale e Colture Cellulari, presso Ospedale S. Camillo de Lellis-Chieti (Supervisore: Prof. Raffaele De Caterina)

4 Basic laboratory techniques; handling and use of laboratory animals (rats, mice); isolation of smooth muscles cells and endothelial cells from human umbilical cord, cell culture of endothelial cells, fibroblasts, smooth muscle cells, cardiomyocytes and cardiomyoblasts; spectrophotometric assays, affinity chromatography; cell surface enzyme immunoassays (EIA), cell adhesion assay, ELISA; Study of genetic polymorphisms by PCR and restriction fragment analysis; Radiometric assays of nitric oxide synthases; Colorimetric assay of nitrate and nitrite. Molecular biology techniques: DNA and RNA extractions, gel electrophoresis, Southern blotting, Western blotting, radioactive and chemiluminescent Northern Blotting, reversetranscriptase-pcr, real-time-pcr, long-pcr, primer design, SDS-PAGE, Immunoblotting, in situ hybridization; elettrophoretic mobility shift assay. Immunocytochemistry, Immunohistochemistry, direct and indirect immunofluorescence; Flow cytometry, flow cell sorting; Confocal microscopy. University fo Texas Medical School and Texas Heart Institute at Houston, TX, Dept. of Internal Medicine, Heart Failure laboratory (Supervisore: Prof. Yong-Jian Geng) Embryonic stem cells, bone marrow stem cells, adipose-derived stem cells; Preparation of red-green fluorescent protein expressing adult and embryonic stem cells; Genetic engeeniring and gene therapy through bacterial tranfection of adult and embryonic stem cells and mammalian somatic cell lines; Genetic engeeniring and gene therapy through adenoviral transfections of adult stem cells and mammalian somatic cell lines; lipo-tranfesction and electroporetion; cloning and sub-cloning; Telomerase activity assay (TRAP); Terminal restriction fragment (TRF) analysis; Langendorff perfusion system and working heart preparation. Lingue conosciute: Italiano, Inglese, Francese, comprensione dello Spagnolo Most relevant publications: ABSTRACTS: 1. M Soccio, T Giacchetti, R Madonna, F Romanazzi, A Di Crecchio, P Di Napoli, S Gallina, A M Calafiore, A Barsotti: Coronary Artery Disease and PVUII Polymorphism of the Lipoprotein Lipasi Gene. JACC 1998, 31 suppl C. 2. M Soccio, R Madonna, A Taccardi, G Vianale, P Di Napoli, M Zimarino, S Gallina, A Procopio, A M Calafiore, A Barsotti: Genetic polymorphism (C 677 T) of methylenetetrahydrofolate reductase gene and coronary heart disease. Int Med Clin Lab 1999, 7 suppl M Soccio, R Madonna, G D Apolito, G Vianale, P Di Napoli, M Zimarino, S Gallina, A Procopio, A M Calafiore, A Barsotti: Effect of endothelial nitric oxide sinthase gene polymorphism on acute ischemic heart disease. Int Med Clin Lab 1999, 7 suppl M. Soccio, R. Madonna, S. Gallina, AM. Calafiore, A. Barsotti, R. De Caterina: A protective effect of the C 242 T of P22PHOX from coronary artery disease. Eur Heart J 2000, 21: S M Massaro, A Zampolli, R Madonna, MA Carluccio, C Storelli, R De Caterina: Statins inhibits cyclo-oxygenase-2 protein and activity in human endothelial cells. Possible contributing to plaque stability. Circulation 2002 november suppl P R Madonna, P Di Napoli, A Grilli, M Felaco, Y-J Geng, R De Caterina. Inhibition of 3-hydroxy-3-methylglutaryl coenzyme A reductase attenuates expression of inducible nitric oxide synthase in cardiac myoblasts FASEB J 2003; 17: R Madonna, A Pandolfi, M Massaro, A Consoli, R De Caterina: insulin enhances vascular cell adhesion molecule (VCAM-1) expression in human cultured endothelial cells. A link to the pathogenesis of accelerated atherosclerosis in diabetes. Journal of American College of Cardiology 2004, march 3 vol. 43 suppl A Li S, Lin J, Lenhean E, Rajabi M, Madonna R, Rosales C, Willerson J T, Geng Y-J: Cardiac response to intracoronarydelivered stem cells in apolipoprotein-null mice: assessment by Langendorff perfusion. Journal of American College of Cardiology 2004, march 3 vol. 43 suppl A Lin J, Li S, Lenhean E, Rajabi M, Madonna R, Rosales C, Willerson J T, Geng Y-J: Ischemic preconditioning improves performance of the hearts with coronary delivery of embryonic stem cells: assessment by a Langendorff perfusion model. Journal of American College of Cardiology 2004, march 3 vol. 43 suppl A Madonna R, Li S, Lin J, Rajabi M, Wassler M J, Geng Y-J: Isolation and characterization of undifferentiated endothelial cell progenitors from murine adipose tissue. FASEB J 2004; 17: R Madonna, R De Caterina, J T Willerson, YJ Geng: Adult Mesenchymal Stem Cells Derived from Adipose Tissue Highly Diabetologia suppl. September 2004; 11. R Madonna, Li S, Lin J, Rajabi M, Wassler M J, R De Caterina, Geng Y-J: Characterization and Heart Trasfusion of Endothelial Progenitor Cells isolated from Adipose Tissue. Eur Heart J suppl august R Madonna, R De Caterina, M J Wassler, YJ Geng: Telomerase activity and expression in adipose tissue-derived stromal cells Evidences for the existence of a more juvanile phenotype in adipose tissue. Eur Heart J suppl august E P Perin, G V Silva, J A Assad, R Madonna, A L Souza, R Majano, A Civitello, J Feldman, J Ober, W K Vaughn, R V Branco, E M Oliveira, L Carvahlo, Y J Geng: Sirolimus-Eluting stent (SES) inhibits growth factor expression and cell proliferation without worsening endothelial function in a pig coronary restenosis model. Circulation 2004, 110: 2870

5 BOOK CHAPTERS: 1. Madonna, G Torge, P Di Napoli, R De Caterina: La Sindrome da basse HDL. In: "I Fattori di Rischio cardiovascolare, Editori: A. L Abbate e R. De Caterina, Edizioni: I quaderni del CNR, Pisa 2. A Zampolli, R Madonna, R De Caterina: Altri fattori di rischio. In: "I Fattori di Rischio cardiovascolare, Editori: A. L Abbate e R. De Caterina, Edizioni: I quaderni del CNR, Pisa; IN EXTENSU PUBLICATIONS 1. Soccio, S Gallina, R Madonna, P Di Napoli, A Barsotti: Apoliproprotein gene abnormalities: molecular basis of coronary heart disease susceptibility. Int Med Clin Lab 1999, 7 suppl 1: S34-S R De Caterina, G Basta, R Madonna, D Statile, M Zimarino, S Gallina, A D Procopio: Endothelial cell transfections: a way for studying promoter activity of genes involved in atherogenesis. Ital Heart J 2000, 1 suppl 3: S De Caterina, R Madonna, J Hassan, A Procopio: Nutrient and gene expression. World Rev Nutr Diet 2001; 89: R De Caterina, R Madonna: Antiarrhythmic effects of omega-3 fatty acids. A review. Ital Heart J Mar;3(3 Suppl): R De Caterina, R Madonna, R Zucchi, M T La Rovere: Antiarrhythmic effects of omega-3 fatty acids: from epidemiology to bedside. Am Heart J 2003: 146, R Madonna, A Pandolfi, M Massaro, A Consoli, R De Caterina: Insulin enhances vascular cell adhesion molecule (VCAM-1) expression in human cultured endothelial cells through the prevalence of a pro-atherogenic p38map-kinase-mediated pathway. Diabetologia 2004; Mar 47 (3): De Caterina R, Zampolli A, Madonna R, Fioretti P, Vanuzzo D: New cardiovascular risk factor: homocysteine and vitamins involved in homocysteine metabolism. Ital Heart J 2004, Suppl 6: 19S-24S 8. De Caterina R, Madonna R, Massaro M: Effects of omega-3- fatty acids on cytokines and adhesion molecules. Curr Atheroscl Rep, : De Caterina R, Madonna R: Nutrients and gene expression. World Rev Nutr Diet 2004, 93: R Madonna, P Di Napoli, M Massaro, A Grilli, M Felaco, A De Caterina, T Daming, R De Caterina, YJ Geng: Statin inhibition of 4-hydroxy-3-methyl glutaryl Coenzyme A (HMG CoA) reductase attenuates expression of cytokineinducible nitric oxide synthase in embryonic cardiac myoblasts. Journal of Biology and Chemistry, manoscritto sottomesso, in revisione 11. R Madonna, J T Willerson, YJ Geng: Adult mesenchymal stem cells derived from adipose tissue highly express telomerase and spontaneously differentiate into contractile cardiomyocytes. Manoscritto sottomesso 12. R Madonna, Li S, Lin J, Rajabi M, Wassler M J, Geng YJ: Expression of nitric oxide synthase during differentiation of telomerase expressing endothelial cells progenitor derived from adipose tissue. Manoscritto sottomesso. 13. E P Perin, G V Silva, J A Assad, R Madonna, A L Souza, R Majano, A Civitello, J Feldman, J Ober, W K Vaughn, R V Branco, E M Oliveira, L Carvahlo, Y J Geng: Sirolimus-Eluting stent (SES) inhibits growth factor expression and cell proliferation without worsening endothelial function in a pig coronary restenosis model. Circulation, manoscritto sottomesso in revisione.